8BCK

Recombinant Tipula oleracea Nudivirus polyhedrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-04
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.5498
Spacegroup name	P 32 2 1
Unit cell lengths	53.772, 53.772, 105.782
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.570 - 1.960
R-factor	0.1886
Rwork	0.184
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bc5
RMSD bond length	0.004
RMSD bond angle	0.625
Data reduction software	xia2.multiplex
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.570	2.030
High resolution limit [Å]	1.960	1.960
Number of reflections	13279	1297
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	160.7
CC(1/2)	0.998	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		301	The protein crystallises in cells.