8BC7

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex an aminoglutarimide degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-23
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.606, 59.450, 89.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.446 - 1.719
Rwork	0.174
R-free	0.21110
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4v2y
RMSD bond length	0.013
RMSD bond angle	1.760
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.446	1.820
High resolution limit [Å]	1.719	1.720
Rmerge	0.104	1.910
Number of reflections	32675	5210
<I/σ(I)>	12.2	1.18
Completeness [%]	100.0	100
Redundancy	13.05	13.54
CC(1/2)	0.999	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.5 M (NH4)H2PO4