8BC6

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex an aspartimide degron peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.966, 59.015, 88.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.230 - 1.720
R-factor	0.196
Rwork	0.195
R-free	0.22261
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4v2y
RMSD bond length	0.012
RMSD bond angle	1.753
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.230	1.820
High resolution limit [Å]	1.718	1.720
Rmerge	0.074	1.749
Number of reflections	32513	5140
<I/σ(I)>	19.8	1.34
Completeness [%]	99.6	99.4
Redundancy	12.73	12.46
CC(1/2)	1.000	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.5 M (NH4)H2PO4