8BC2
Ligand-Free Structure of the decameric sulfofructose transaldolase BmSF-TAL
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.6 |
| Number of Particle | 153063 |
| Software | RELION,EPU |
| Symmetry | D5 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
