8BBT

Native Tipula oleracea Nudivirus polyhedrin - 1960

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE VMXm
Synchrotron site	Diamond
Beamline	VMXm
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.63282
Spacegroup name	P 32 2 1
Unit cell lengths	53.389, 53.389, 105.578
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.000 - 1.690
R-factor	0.1911
Rwork	0.189
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bc5
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	xia2.multiplex
Data scaling software	xia2.multiplex
Phasing software	CRANK
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	1.750
High resolution limit [Å]	1.690	1.690
Rmerge	0.793
Number of reflections	19056	1962
<I/σ(I)>	9.91
Completeness [%]	94.0
Redundancy	19.6
CC(1/2)	0.967	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		293	Protein natively forms crystals inside insect cells.