8BBR
Determination of the structure of active tyrosinase from bacterium Verrucomicrobium spinosum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97626 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.298, 63.443, 117.311 |
Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
Resolution | 116.370 - 1.640 |
R-factor | 0.1922 |
Rwork | 0.190 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4z11 4z12 5ce9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.586 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 116.370 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 75699 | 3073 |
<I/σ(I)> | 9.8 | |
Completeness [%] | 98.2 | |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.362 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M ammonium sulfate, 0.1 M MES pH 6.5, 30% w/v PEG5000MME |