8BBN

SARS-CoV-2 Delta-RBD complexed with BA.2-10 and EY6A Fabs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97628
Spacegroup name	P 21 21 21
Unit cell lengths	170.655, 171.691, 176.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.790 - 3.580
R-factor	0.268
Rwork	0.265
R-free	0.31390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7nx6
RMSD bond length	0.002
RMSD bond angle	0.523
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.000	3.650
High resolution limit [Å]	3.580	3.580
Rmerge	0.467
Rpim	0.130
Number of reflections	61670	2966
<I/σ(I)>	4.1	0.3
Completeness [%]	100.0	97.2
Redundancy	13.8
CC(1/2)	0.992	0.301

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% (v/v) 2-propanol, 0.1 M BICINE, pH 8.5 and 30% (w/v) PEG 1500