8BBM
DNA binding domain of J-DNA Binding Protein 1 (JBP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 68.286, 68.286, 185.842 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.590 - 1.950 |
| R-factor | 0.20453 |
| Rwork | 0.202 |
| R-free | 0.25194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xse |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.621 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmeas | 0.157 | 5.033 |
| Number of reflections | 19605 | 53016 |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 38.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15-17% Peg 6000, 0.1M Sodium iodide or 15-17% Peg, 0.2M potassium nitrate |
