8BB7

Crystal structure of Mouse Plexin-B1 (20-535) in complex with VHH15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	93.544, 108.988, 94.043
Unit cell angles	90.00, 90.37, 90.00

Refinement procedure

Resolution	47.150 - 2.950
R-factor	0.2039
Rwork	0.203
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol2
RMSD bond length	0.007
RMSD bond angle	1.107
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.194	47.150	3.190
High resolution limit [Å]	2.948	10.570	3.050
Rmerge	0.420	0.087	2.358
Rmeas	0.504	0.104	2.815
Rpim	0.274	0.057	1.520
Number of reflections	36092	896	4383
<I/σ(I)>	5.6
Completeness [%]	99.9
Redundancy	6.4	5.9	6.6
CC(1/2)	0.953	0.992	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	8% (w/v) PEG 8000, 0.1 M Sodium Citrate, pH 5.0, 1% (w/v) dextran-sulphate (Mr 5000)