8BB6

Crystal structure of Arabidopsis thaliana sucrose transporter SUC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	58.776, 65.622, 82.813
Unit cell angles	89.96, 101.74, 94.72

Refinement procedure

Resolution	34.740 - 2.680
R-factor	0.2711
Rwork	0.270
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RoseTTAFold predicted model
Data reduction software	DIALS (3.3.2)
Data scaling software	xia2 (3.3.2)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.350	57.360	2.730
High resolution limit [Å]	2.680	7.270	2.680
Rmeas	0.314	0.085	1.985
Rpim	0.171	0.047	1.073
Total number of observations	110948	5412	5385
Number of reflections	33404	1697	1586
<I/σ(I)>	4.7	19.5	0.8
Completeness [%]	98.6	99.7	95.1
Redundancy	3.3	3.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.3	292	43.5% PEG 400, 0.25 M Ammonium dihydrogen phosphate, 0.15 M BIS-TRIS propane