8BAP

Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, eightfold mutant active on propanol syringol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000002
Spacegroup name	P 1
Unit cell lengths	113.556, 142.995, 154.600
Unit cell angles	114.87, 97.00, 93.28

Refinement procedure

Resolution	49.044 - 2.300
R-factor	0.1956
Rwork	0.193
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fxd
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.137	0.707
Number of reflections	379398	18712
<I/σ(I)>	4.6	1.1
Completeness [%]	98.2	97.6
Redundancy	3.5
CC(1/2)	0.987	0.537

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Morpheus screening, condition A6