Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

8BAO

Dysgonamonadaceae bacterium CRISPR ancillary nuclease 2

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04
Synchrotron siteDiamond
BeamlineI04
Temperature [K]100
Detector technologyPIXEL
Collection date2020-10-07
DetectorDECTRIS EIGER2 XE 16M
Wavelength(s)0.9795
Spacegroup nameC 1 2 1
Unit cell lengths155.133, 109.429, 63.043
Unit cell angles90.00, 101.88, 90.00
Refinement procedure
Resolution54.710 - 2.060
R-factor0.2216
Rwork0.220
R-free0.25440
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)AlphaFold
RMSD bond length0.008
RMSD bond angle0.954
Data reduction softwareXDS
Data scaling softwarepointless
Phasing softwarePHASER
Refinement softwarePHENIX (1.20.1_4487)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]54.7152.093
High resolution limit [Å]2.0582.058
Rmerge0.2402.405
Rmeas0.2602.593
Rpim0.1000.963
Number of reflections638043196
<I/σ(I)>5.10.9
Completeness [%]100.0100
Redundancy6.8
CC(1/2)0.9930.342
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.52870.1M Imidazole; MES monohydrate (acid) 0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide 20% v/v Glycerol; 10% w/v PEG 4000 208.3uM cA4

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon