8BAO
Dysgonamonadaceae bacterium CRISPR ancillary nuclease 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.133, 109.429, 63.043 |
Unit cell angles | 90.00, 101.88, 90.00 |
Refinement procedure
Resolution | 54.710 - 2.060 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold |
RMSD bond length | 0.008 |
RMSD bond angle | 0.954 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.715 | 2.093 |
High resolution limit [Å] | 2.058 | 2.058 |
Rmerge | 0.240 | 2.405 |
Rmeas | 0.260 | 2.593 |
Rpim | 0.100 | 0.963 |
Number of reflections | 63804 | 3196 |
<I/σ(I)> | 5.1 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.8 | |
CC(1/2) | 0.993 | 0.342 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287 | 0.1M Imidazole; MES monohydrate (acid) 0.03M Sodium fluoride; 0.03M Sodium bromide; 0.03M Sodium iodide 20% v/v Glycerol; 10% w/v PEG 4000 208.3uM cA4 |