8B9X

Chimeric protein of human UFM1 E3 ligase, UFL1, and DDRGK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-20
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 64
Unit cell lengths	145.568, 145.568, 83.175
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.112 - 3.066
Rwork	0.233
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold model
RMSD bond length	0.015
RMSD bond angle	1.744
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.112	3.280
High resolution limit [Å]	3.066	3.066
Number of reflections	18967	3414
<I/σ(I)>	8.7
Completeness [%]	99.8
Redundancy	5.9
CC(1/2)	0.997	0.446

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Sodium acetate trihydrate Ammonium tartrate dibasic