8B9W

Cysteine Synthase from Trypanosoma theileri with PLP bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	I 2 3
Unit cell lengths	187.303, 187.303, 187.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.466 - 2.750
Rwork	0.148
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4air
RMSD bond length	0.014
RMSD bond angle	2.246
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.470	2.800
High resolution limit [Å]	2.750	2.750
Number of reflections	28493	1434
<I/σ(I)>	23.5
Completeness [%]	100.0
Redundancy	56.3
CC(1/2)	0.997	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, Sodium cacodylate