8B9O

Structure of the C-terminal domain of ClpC2 from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	51.080, 40.630, 64.430
Unit cell angles	90.00, 91.75, 90.00

Refinement procedure

Resolution	32.200 - 2.000
R-factor	0.2064
Rwork	0.205
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdb
RMSD bond length	0.004
RMSD bond angle	0.611
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.060
High resolution limit [Å]	2.000	2.000
Rmeas	0.550	0.981
Number of reflections	8941	610
<I/σ(I)>	14.9
Completeness [%]	98.9	89.7
Redundancy	4.3	3.9
CC(1/2)	0.998	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) MPD, 0.02 M of each amino acid and 0.1 M MES/imidazole pH 6.5