8B9O
Structure of the C-terminal domain of ClpC2 from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 51.080, 40.630, 64.430 |
Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
Resolution | 32.200 - 2.000 |
R-factor | 0.2064 |
Rwork | 0.205 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wdb |
RMSD bond length | 0.004 |
RMSD bond angle | 0.611 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20_4459: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.060 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.550 | 0.981 |
Number of reflections | 8941 | 610 |
<I/σ(I)> | 14.9 | |
Completeness [%] | 98.9 | 89.7 |
Redundancy | 4.3 | 3.9 |
CC(1/2) | 0.998 | 0.669 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) MPD, 0.02 M of each amino acid and 0.1 M MES/imidazole pH 6.5 |