8B8V
Crystal structure of the Rabies virus RNA free nucleoprotein- phosphoprotein complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.872900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.571, 73.617, 154.361 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.1807 |
| Rwork | 0.178 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gtt |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.778 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.530 |
| Number of reflections | 19735 | 1397 |
| <I/σ(I)> | 12.31 | 3.76 |
| Completeness [%] | 99.8 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.02M sodium/potassium phosphate, 0.1M Bis Tris propane pH 6.5, 20% w/v PEG 3350 |
