8B8O
Crystal structure of Scribble PDZ1 with human papillomavirus strain 16 E6 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.091, 90.992, 36.356 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 32.030 - 2.900 |
| R-factor | 0.2705 |
| Rwork | 0.270 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6xa6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.662 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.030 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.144 | 1.293 |
| Number of reflections | 4654 | 451 |
| <I/σ(I)> | 8.41 | 0.93 |
| Completeness [%] | 99.6 | |
| Redundancy | 7 | |
| CC(1/2) | 0.996 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293.15 | 0.1 M Na HEPES pH 7.9, 63% v/v MPD |
