8B8I
Nanobody (NbLumSyt1) bound to human Syt1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-29 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.955, 100.226, 103.939 |
Unit cell angles | 90.00, 100.83, 90.00 |
Refinement procedure
Resolution | 59.110 - 2.750 |
Rwork | 0.263 |
R-free | 0.29770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i2g |
RMSD bond length | 0.007 |
RMSD bond angle | 1.185 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.570 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 46246 | 4523 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.6 M DL-malic acid |