8B8F
Atomic structure of the beta-trefoil domain of the Laccaria bicolor lectin LBL in complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.530, 61.270, 44.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.200 - 1.000 |
| R-factor | 0.1408 |
| Rwork | 0.140 |
| R-free | 0.15230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y9f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.269 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.200 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rpim | 0.044 | 0.044 |
| Number of reflections | 79659 | 11493 |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | |
| CC(1/2) | 0.988 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (w/v) PEG 6000, 0.1 M sodium acetate, pH 5.0, and 0.2 M ammonium chloride |
