8B8D

multimerization domain of Gaboon Viper Virus 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 21 21 2
Unit cell lengths	81.824, 82.499, 89.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 2.400
R-factor	0.2329
Rwork	0.228
R-free	0.31060
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.421
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.000	2.870	2.460
High resolution limit [Å]	2.400	2.790	2.400
Rmerge	0.114
Rpim	0.046
Number of reflections	24108	988	1742
<I/σ(I)>	11.4
Completeness [%]	99.6	89.3	99.4
Redundancy	13.5	10	13.9
CC(1/2)	0.999	0.657	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES pH 7.5, 18-22 % PEG 3350, 200 mM NaSCN
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM HEPES pH 7.5, 18-22 % PEG 3350, 200 mM NaSCN