8B8B
Multimerization domain of Munia virus 1 phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.226, 42.746, 72.195 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.150 |
| R-factor | 0.2238 |
| Rwork | 0.220 |
| R-free | 0.31590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292125760 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.655 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.052 | 0.670 |
| Rpim | 0.037 | 0.471 |
| Number of reflections | 23663 | 2043 |
| <I/σ(I)> | 15.2 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.6 |
| CC(1/2) | 0.998 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15-18 % PEG 3350, 150 mM Mg(NO3)2, 10 mM phenol |
