8B87
Crystal structure of Scribble PDZ1 with human papillomavirus strain 16 E6 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95372 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.620, 31.140, 91.760 |
Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
Resolution | 35.570 - 2.000 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mtv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.470 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.570 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.836 |
Number of reflections | 13792 | 1385 |
<I/σ(I)> | 8.11 | |
Completeness [%] | 98.9 | |
Redundancy | 2.9 | |
CC(1/2) | 0.998 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M Sodium Fluoride, 0.1 M Bis-Tris propane pH 6.5, 20% w/v PEG 3350 |