8B80
The structure of Gan1D W433A in complex with galactose-6P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 107.500, 68.600, 152.290 |
Unit cell angles | 90.00, 99.62, 90.00 |
Refinement procedure
Resolution | 19.932 - 1.780 |
R-factor | 0.1792 |
Rwork | 0.178 |
R-free | 0.21640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5okh |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.084 | 0.944 |
Number of reflections | 104900 | 16376 |
<I/σ(I)> | 13.97 | |
Completeness [%] | 99.4 | |
Redundancy | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 19% polyethylene glycol 8 K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |