8B80
The structure of Gan1D W433A in complex with galactose-6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.500, 68.600, 152.290 |
| Unit cell angles | 90.00, 99.62, 90.00 |
Refinement procedure
| Resolution | 19.932 - 1.780 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5okh |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.084 | 0.944 |
| Number of reflections | 104900 | 16376 |
| <I/σ(I)> | 13.97 | |
| Completeness [%] | 99.4 | |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 19% polyethylene glycol 8 K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |
