8B73
Acetivibrio clariflavus beta-1,4-xylanase of Glycoside Hydrolase Family 10 (AcXyn10A).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-07 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.566, 88.916, 173.479 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.089 - 2.240 |
Rwork | 0.206 |
R-free | 0.23160 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.950 |
Data reduction software | xia2 |
Data scaling software | xia2.multiplex |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.580 | 2.290 |
High resolution limit [Å] | 2.240 | 2.240 |
Rmerge | 0.166 | 2.685 |
Rmeas | 0.173 | |
Rpim | 0.050 | 0.764 |
Number of reflections | 61326 | 3029 |
<I/σ(I)> | 8 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 12.7 | 13.5 |
CC(1/2) | 0.997 | 0.405 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293.15 | H6 conditions of JCSG+ HTS 0.1 M Ammonium acetate 0.1 M BIS-Tris, 17 % w/v PEG 10,000 109.79mM Xyltetraose (10X) with AcXynA protein in buffer containing 50mM NaCl, 20mM Tris-Cl |