8B6E
crystal structure of the DNA-binding short chromatophore-targeted protein sCTP-23166 from Paulinella chromatophora
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9200 |
Spacegroup name | P 1 |
Unit cell lengths | 28.887, 30.943, 31.188 |
Unit cell angles | 83.99, 63.45, 69.84 |
Refinement procedure
Resolution | 29.000 - 1.200 |
R-factor | 0.1333 |
Rwork | 0.131 |
R-free | 0.17630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | alphafold |
RMSD bond length | 0.015 |
RMSD bond angle | 1.993 |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.243 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.070 | |
Number of reflections | 27013 | 2610 |
<I/σ(I)> | 12.04 | |
Completeness [%] | 93.0 | |
Redundancy | 3.6 | |
CC(1/2) | 0.997 | 0.945 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1 M sodium HEPES pH 7.5, 15 % (w/v) PEG 20000 |