8B5S
Crystal Structure of P. aeruginosa WaaG in complex with UDP-glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.633, 94.426, 70.170 |
Unit cell angles | 90.00, 101.41, 90.00 |
Refinement procedure
Resolution | 57.540 - 1.950 |
R-factor | 0.18173 |
Rwork | 0.180 |
R-free | 0.21905 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2iv7 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.870 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.551 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 182700 | 3796 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.979 | 0.979 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.06M Divalents, 0.1M Buffer System 3 pH 8.5, 30.0% v/v P500MME_P20K |