8B4N

X-ray structure of phycoerythrin from Porphyridium cruentum

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	H 3
Unit cell lengths	186.590, 186.590, 59.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.648 - 1.600
Rwork	0.222
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v58
RMSD bond length	0.010
RMSD bond angle	1.602
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.648	1.625
High resolution limit [Å]	1.597	1.597
Rmerge	0.101	1.028
Rmeas	0.107	0.999
Rpim	0.035	0.360
Number of reflections	100909	44973
<I/σ(I)>	12.9	2.2
Completeness [%]	99.0	98.3
Redundancy	9.2	9
CC(1/2)	0.997	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PE crystals were grown by hanging drop vapor diffusion method (Russo Krauss et al., 2013) using a drop containing 10 mg/mL PE in 0.250 M ammonium sulphate, 25.0 mM potassium phosphate at pH 5.0, equilibrated with a reservoir containing 0.500 M ammonium sulphate, 50.0 mM potassium phosphate at pH 5.0.