8B4K
Rfa1-N-terminal domain in complex with phosphorylated Ddc2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-26 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.506, 34.600, 99.350 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 54.510 - 1.550 |
R-factor | 0.1692 |
Rwork | 0.168 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5omb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.361 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.510 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.039 | 0.292 |
Number of reflections | 54483 | 5391 |
<I/σ(I)> | 19.8 | |
Completeness [%] | 99.6 | 98.9 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 0.2M Lithium chloride, 0.1M Sodium acetate, pH 5.0, 20% w/v PEG 6000 |