8B46

Crystal structure of the SUN1-KASH6 9:9 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.6702
Spacegroup name	P 63
Unit cell lengths	134.094, 134.094, 106.586
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.890 - 1.670
R-factor	0.1669
Rwork	0.166
R-free	0.18960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r2i
RMSD bond length	0.014
RMSD bond angle	1.296
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.129	1.889
High resolution limit [Å]	1.665	1.665
Rmerge	0.133	1.887
Rpim	0.043	0.622
Number of reflections	70755	3538
<I/σ(I)>	17.4	1.9
Completeness [%]	55.7	8.9
Redundancy	21.1
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M Magnesium chloride, 0.1 M MES pH 6.0, 3% w/v PEG 6000 (+25% ethylene glycol cryoprotectant)