8B3Z
SigE N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.953, 165.143, 98.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.390 - 2.380 |
| R-factor | 0.2009 |
| Rwork | 0.198 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ugo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.571 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.0.32) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.390 | 2.500 |
| High resolution limit [Å] | 2.379 | 2.379 |
| Rmerge | 0.126 | |
| Rmeas | 0.131 | |
| Number of reflections | 27360 | 1349 |
| <I/σ(I)> | 13.4 | 1.8 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 13 | 12.3 |
| CC(1/2) | 0.999 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.5 M Potassium thiocyanate 0.1 M Tris pH 8.5 7 mg.mL-1 in a 1:1 ratio |
