8B3S

Structure of YjbA in complex with ClpC N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-17
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9763
Spacegroup name	P 31
Unit cell lengths	69.713, 69.713, 89.601
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.867 - 2.090
R-factor	0.1871
Rwork	0.185
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hbn
RMSD bond length	0.002
RMSD bond angle	0.514
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	2.130
High resolution limit [Å]	2.090	2.090
Number of reflections	28876	1440
<I/σ(I)>	9.8
Completeness [%]	100.0	100
Redundancy	8.6	8.8
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	4% PEG 8000, 0.2 M Magnesium Chloride