8B3S
Structure of YjbA in complex with ClpC N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-17 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 |
| Unit cell lengths | 69.713, 69.713, 89.601 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.867 - 2.090 |
| R-factor | 0.1871 |
| Rwork | 0.185 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.16_3549: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 2.130 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 28876 | 1440 |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.8 |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 4% PEG 8000, 0.2 M Magnesium Chloride |
