8B3E

Variant Surface Glycoprotein VSG397

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	50.557, 218.484, 66.436
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.760 - 1.260
R-factor	0.1845
Rwork	0.183
R-free	0.21580
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.760	1.305
High resolution limit [Å]	1.260	1.260
Number of reflections	96793	8421
<I/σ(I)>	6.56
Completeness [%]	96.4
Redundancy	5.5
CC(1/2)	0.990	0.219

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	296	100 mM SPG Buffer, 21 % (WV) PEG 1500