8B1O
Crystal structure of SUDV VP40 C314S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.999998 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.247, 91.005, 48.433 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 48.160 - 1.750 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ld8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 29723 | 1486 |
| <I/σ(I)> | 10.1 | 1.9 |
| Completeness [%] | 98.5 | 91.6 |
| Redundancy | 3.8 | 3.2 |
| CC(1/2) | 0.994 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus A7 30% (w/v) GOL_P4K (glycerol and PEG4000) 0.1 M morpheus buffer 2 (HEPES and MOPS) 60 mM morpheus divalents (MgCl2, CaCl2) |
