8B1O
Crystal structure of SUDV VP40 C314S mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999998 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 64.247, 91.005, 48.433 |
Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
Resolution | 48.160 - 1.750 |
R-factor | 0.1874 |
Rwork | 0.186 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ld8 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.793 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 29723 | 1486 |
<I/σ(I)> | 10.1 | 1.9 |
Completeness [%] | 98.5 | 91.6 |
Redundancy | 3.8 | 3.2 |
CC(1/2) | 0.994 | 0.803 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus A7 30% (w/v) GOL_P4K (glycerol and PEG4000) 0.1 M morpheus buffer 2 (HEPES and MOPS) 60 mM morpheus divalents (MgCl2, CaCl2) |