8B0U

Structure of the CalpL/T10 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	89.754, 96.598, 131.710
Unit cell angles	90.00, 98.19, 90.00

Refinement procedure

Resolution	49.310 - 3.290
R-factor	0.197
Rwork	0.195
R-free	0.23010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qda
RMSD bond length	0.002
RMSD bond angle	0.533
Phasing software	PHASER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	3.408
High resolution limit [Å]	3.290	3.290
Number of reflections	33777	3321
<I/σ(I)>	9.92
Completeness [%]	99.1	99.34
Redundancy	6.8	6.9
CC(1/2)	0.997	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M ammonium sulfate, 0.1 M ammonium cacodylate, 0.2 M NaCl at pH 6.5