8AYT

Crystal structure of SUDV VP40 W95A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000009
Spacegroup name	C 1 2 1
Unit cell lengths	64.752, 91.279, 48.934
Unit cell angles	90.00, 96.71, 90.00

Refinement procedure

Resolution	45.640 - 1.900
R-factor	0.1685
Rwork	0.167
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ld8
RMSD bond length	0.007
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	1.940
High resolution limit [Å]	1.900	1.900
Number of reflections	22067	1406
<I/σ(I)>	10.7	2.5
Completeness [%]	99.1	98.2
Redundancy	3.8	3.6
CC(1/2)	0.990	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M potassium chloride 50 mM MOPS 12% (w/v) PEG4000 20% (w/v) glycerol