8AYR

Sialidases and Fucosidases of Akkermansia muciniphila are key for rapid growth on colonic mucin and nutrient sharing amongst mucin-associated human gut microbiota

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-15
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.976300
Spacegroup name	P 1
Unit cell lengths	76.190, 76.450, 84.500
Unit cell angles	88.41, 89.08, 88.23

Refinement procedure

Resolution	34.510 - 2.700
R-factor	0.2353
Rwork	0.233
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	alpha fold
RMSD bond length	0.005
RMSD bond angle	0.862
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.510	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.082	0.745
Rmeas	0.095	0.812
Rpim	0.048
Number of reflections	99265	2720
<I/σ(I)>	9.93
Completeness [%]	90.5	71
Redundancy	3.8	3.5
CC(1/2)	0.998	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.7	293	0.1 M Hepes, 16% PEG10k,