8AYC
Scalindua brodae amxFabZ, I69G mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 104.598, 104.598, 79.797 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.390 - 1.750 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.20210 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u1z |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.390 | 1.817 |
| High resolution limit [Å] | 1.746 | 1.746 |
| Rmerge | 0.042 | 0.876 |
| Number of reflections | 15812 | 793 |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 92.2 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.999 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.0 M Sodium formate |
