8AY2
Crystal structure of the C-terminal part of rat Sec8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97944 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 190.004, 190.004, 175.275 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.500 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.21490 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.746 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.990 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.134 | |
Rmeas | 0.138 | |
Rpim | 0.035 | |
Number of reflections | 94874 | 2815 |
<I/σ(I)> | 20.47 | |
Completeness [%] | 89.2 | |
Redundancy | 14.9 | |
CC(1/2) | 0.999 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 297 | 0.1M 2-(N-morpholino)ethanesulfonic acid, 1.5-2M NaCl, 10-15% (v/v) PEG6000, 2mM MgCl2, 10mM DTT |