8AXY
Staphylococcus aureus endonuclease IV orthorhombic crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.800 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.857, 82.581, 105.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.545 - 1.050 |
Rwork | 0.136 |
R-free | 0.15770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xp3 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.566 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | MoRDa |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.545 | 1.070 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.063 | 1.882 |
Number of reflections | 156665 | 7372 |
<I/σ(I)> | 14.2 | 0.5 |
Completeness [%] | 99.1 | 95.8 |
Redundancy | 7.2 | 6.1 |
CC(1/2) | 1.000 | 0.319 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | - 0.1 M HEPES pH 7.0 - 2.2 M Ammonium sulfate |