8AXO

Crystal structure of the C-terminal domain of Trypanosoma brucei CFAP410

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 32 2 1
Unit cell lengths	40.107, 40.107, 62.684
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.100 - 1.290
R-factor	0.1882
Rwork	0.186
R-free	0.20740
Structure solution method	AB INITIO PHASING
RMSD bond length	0.005
RMSD bond angle	0.611
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.100	1.340
High resolution limit [Å]	1.290	1.290
Rmerge	0.132
Rmeas	0.140
Rpim	0.046
Number of reflections	15025	1430
<I/σ(I)>	8.16
Completeness [%]	99.7
Redundancy	9.4
CC(1/2)	0.991	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	2.0 M ammonium sulfate, 5% (v/v) iso-propanol