8AX4
Crystal structure of FMV N in its RNA-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.072 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 99.870, 96.920, 103.520 |
Unit cell angles | 90.00, 111.92, 90.00 |
Refinement procedure
Resolution | 48.460 - 2.280 |
R-factor | 0.2168 |
Rwork | 0.217 |
R-free | 0.22040 |
Structure solution method | SIRAS |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.460 | 48.460 | 2.340 |
High resolution limit [Å] | 2.280 | 10.200 | 2.280 |
Rmerge | 0.067 | 0.012 | 1.618 |
Rmeas | 0.079 | 0.014 | 1.897 |
Total number of observations | 296115 | ||
Number of reflections | 82002 | 953 | 6021 |
<I/σ(I)> | 15.31 | 76.99 | 0.94 |
Completeness [%] | 98.8 | 96.1 | 99.2 |
Redundancy | 3.611 | 3.687 | 3.643 |
CC(1/2) | 0.999 | 1.000 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 3.45 mM Sodium Formate |