8AWS
Millisecond cryo-trapping by the spitrobot crystal plunger, Xylose Isomerase with Glucose at 50ms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2022-02-22 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 93.181, 98.644, 102.639 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.990 - 2.260 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rnf |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.990 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.273 | |
Number of reflections | 17488 | 800 |
<I/σ(I)> | 8.6 | |
Completeness [%] | 84.3 | |
Redundancy | 12.2 | |
CC(1/2) | 0.993 | 0.559 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 |