8AWM
RVFV GnH with Fab268 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.072, 98.587, 123.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.570 - 3.500 |
| R-factor | 0.2482 |
| Rwork | 0.247 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f8p |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.570 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.560 | 2.004 |
| Rpim | 0.569 | |
| Number of reflections | 10540 | 558 |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13 | |
| CC(1/2) | 0.992 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M bis-tris pH 5.5, 25% w/v PEG 3,350 |
