8AWI
Crystal structure of Human Transthyretin at 1.15 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97993 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 42.853, 64.325, 85.863 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.325 - 1.150 |
R-factor | 0.14846 |
Rwork | 0.147 |
R-free | 0.16580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | in-house model |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.325 | 1.170 |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 84053 | 39058 |
<I/σ(I)> | 19.5 | |
Completeness [%] | 98.8 | |
Redundancy | 23.1 | |
CC(1/2) | 0.996 | 0.670 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | protein at 5.2mg/ml in 10mM sodium phosphate pH7.6 and 100mM potassium chloride was mixed 1:1 with 1.3-1.6M sodium citrate and 3.5% glycerol |