8AWB
Xylose Isomerase in 90% relative humidity environment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2022-02-16 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 93.839, 99.357, 102.256 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.140 - 2.300 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rnf |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.140 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 18853 | 944 |
<I/σ(I)> | 6.9 | |
Completeness [%] | 81.0 | |
Redundancy | 3.3 | |
CC(1/2) | 0.859 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 293 | 100 mM HEPES 200 mM Lithium Sulphate 35% Peg3350 |