8AW4
Structure of a complex of biosynthetic proteins bB-E3 and bGFPD-YY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2018-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 124.330, 124.330, 110.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.190 - 2.210 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zv6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.120 | |
| Number of reflections | 16555 | 2546 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 98.4 | |
| Redundancy | 10.9 | |
| CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 293 | Tris-HCl, sodium acetate, |
