8AVP
Structure of short biotin complexed agroavidin with the Hoef tail.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.965 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.106, 81.576, 170.050 |
| Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.870 |
| R-factor | 0.23446 |
| Rwork | 0.231 |
| R-free | 0.29716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ast |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.634 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.960 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmerge | 0.089 | 0.932 |
| Number of reflections | 74109 | 4436 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.35 | |
| CC(1/2) | 0.993 | 0.465 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% ethylene glycol 0.05M acetic acid pH 3.2 |
