8AVK
Superoxide dismutase SodFM1 from CPR Parkubacteria Wolfebacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.89843 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.531, 70.586, 57.788 |
Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
Resolution | 56.857 - 2.100 |
Rwork | 0.209 |
R-free | 0.25630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ix9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.827 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.590 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 21632 | 1781 |
<I/σ(I)> | 7.3 | 1 |
Completeness [%] | 100.0 | |
Redundancy | 14.1 | |
CC(1/2) | 0.996 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 100 mM sodium acetate trihydrate pH 4.5 and 25% w/v polyethylene glycol 3350. |