8AVJ
Structure of short apo agroavidin with the Hoef tail.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.965 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 116.697, 116.697, 147.889 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.300 - 2.100 |
R-factor | 0.20398 |
Rwork | 0.203 |
R-free | 0.23029 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8asu |
RMSD bond length | 0.014 |
RMSD bond angle | 1.778 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.134 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.051 | 0.613 |
Number of reflections | 64372 | 16884 |
<I/σ(I)> | 0.998 | |
Completeness [%] | 99.3 | |
Redundancy | 5.2 | |
CC(1/2) | 0.998 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% ethylene glycol 0.05M acetic acid pH 3.2 |