8AU6

Structure of Corynebacterium glutamicum Cg1604, a cell division regulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-28
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979261
Spacegroup name	C 2 2 21
Unit cell lengths	66.780, 105.500, 70.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.040 - 2.000
R-factor	0.1957
Rwork	0.194
R-free	0.22850
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.100	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.035	0.642
Number of reflections	17209	1207
<I/σ(I)>	16.1
Completeness [%]	99.7	95.9
Redundancy	14.6	11.5
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	30 % (w/v) PEG 4K, 0.2 M MgCl2, 0.1 M TRIS pH 8.5